Question 1

Match each term with its definition.
-transgenic

Accepted Answer

A)  uses DNA technologies to alter genes in a cell or organism
B)  branch of biology that tries to combine proteomics and genomics at an organismal level
C)  determination and analysis of the nucleotide sequences in the chromosomes in an organism
D)  products of a restriction endonuclease's action
E)  collection of clones that together contain every DNA sequence in a genome, including noncoding sequencesF) use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify itG) collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell typeH) procedure described as "a photocopy machine for specific DNA sequences"I) DNA from two different sources that have been joined together into a single moleculeJ) term used to describe organisms that have been subject to genetic engineeringK) study of all of the proteins produced from an organism's genomeL) study of the functions of genes and noncoding portions of the genome, including their interactions with each otherM) bacterial enzymes that recognize and cut specific DNA sequencesN) method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresisO) technique used to separate DNA molecules based on their relative sizesP) method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genomeQ) B) and G)
R) C) and G)

Question 2

Which of the following describes biotechnology?

Accepted Answer

A)   any technique applied to dead organisms to make or modify products or processes for a specific purpose.
B)   any technique applied to biological systems or living organisms to make or modify products or processes for a specific purpose
C)   any technique applied to non-biological systems or dead organisms to make or modify products or processes for a specific purpose
D)   any technique applied to non-biological systems to make or modify products or processes for a specific purposeE) A) and B)
F) A) and C)

Question 3

Which of the following best describes the purpose of creating transgenic mice?

Accepted Answer

A)   to study genetic changes in context
B)   to produce proteins
C)   to clone mice
D)   to create antibiotic-resistant miceE) B) and C)
F) None of the above

Question 4

Match each term with the most appropriate macromolecule being studied or manipulate
-cloning

Accepted Answer

A)  DNA
B)  mRNA/cDNA
C)  protein
D)   protein.E) B) and C)
F) All of the above

Question 5

Suppose that you generate cDNA libraries for the same cell line under different conditions. Also suppose that when you compare the libraries, you find that some cDNAs in one library are missing from the other library. What is the best explanation for this result?

Accepted Answer

A)   One of the cDNA libraries is really a genomic library.
B)   There was an error in generating one of the cDNA libraries, and some of the sequences were not cloned properly.
C)   Even the same cell type will change gene expression as conditions change. The difference in cDNAs tells which genes are expressed in each set of conditions.
D)   The probe used in the Southern blot of the libraries must have been poorly constructed.E) A) and D)
F) B) and D)

Question 6

Suppose that you have cloned a genomic library and know that the bacteria have foreign DNA fragments inserted into the cloning vector. Also suppose that you need to identify the specific bacteria that have the gene coding for protein X. How can these bacteria be located?

Accepted Answer

A)   by restriction endonuclease treatment of DNA from some of the bacterial colonies
B)   by DNA hybridization of the different colonies
C)   by agarose gel electrophoresis of DNA isolated from the bacterial colonies
D)   by PCR of the same bacterial mixture that was spread on agarose platesE) B) and C)
F) B) and D)

Question 7

Match each term with its definition.
-systems biology

Accepted Answer

A)  uses DNA technologies to alter genes in a cell or organism
B)  branch of biology that tries to combine proteomics and genomics at an organismal level
C)  determination and analysis of the nucleotide sequences in the chromosomes in an organism
D)  products of a restriction endonuclease's action
E)  collection of clones that together contain every DNA sequence in a genome, including noncoding sequencesF) use of a short single stranded DNA sequence, labelled with some sort of tag, to bind to complementary DNA and thus identify itG) collection of clones that together contain a DNA version of every mRNA sequence expressed in a particular cell typeH) procedure described as "a photocopy machine for specific DNA sequences"I) DNA from two different sources that have been joined together into a single moleculeJ) term used to describe organisms that have been subject to genetic engineeringK) study of all of the proteins produced from an organism's genomeL) study of the functions of genes and noncoding portions of the genome, including their interactions with each otherM) bacterial enzymes that recognize and cut specific DNA sequencesN) method of hybridizing labelled DNA to DNA fragments that were previously subjected to gel electrophoresisO) technique used to separate DNA molecules based on their relative sizesP) method of identifying individuals based on their individual patterns of short tandem repeats located on certain loci of their genomeQ) None of the above
R) I) and K)

Question 8

How are the sticky ends obtained?

Accepted Answer

A)   by using a different restriction enzyme to cut both DNA fragment and the plasmid
B)   by using the same restriction enzyme to cut both DNA fragment and the plasmid
C)   by using a restriction enzyme to cut DNA fragment
D)   by using a restriction enzyme to cut the plasmidE) A) and B)
F) B) and C)

Question 9

Figure 14-1 
-According to Figure 14-1, which of the following is the difference between the original plasmid and the recombinant plasmid?

Accepted Answer

A)   The original plasmid is not found in nature.
B)   The original plasmid has foreign DNA in it.
C)   Recombinant plasmid does not have foreign DNA in it.
D)   Recombinant plasmid has foreign DNA in it.E) None of the above
F) All of the above

Question 10

Why is Southern blotting used?

Accepted Answer

A)   to identify samples with specific DNA sequences
B)   to separate DNA based on size
C)   to amplify a genomic region of interest
D)   to transfer DNA samples to a special membrane or filter paperE) A) and B)
F) A) and C)

Question 11

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all.
-This technique is used to amplify specific sequences of DNA.

Accepted Answer

A)  DNA library
B)  restriction enzyme
C)  gene cloning
D)  Southern blotting
E)  PCRF) D) and E)
G) B) and C)

Question 12

What is a benefit of introducing new genes into a plant?

Accepted Answer

A)   The plant's growth rate reduces.
B)   The plant's genes mutate faster.
C)   The plant's nutritional value to primary consumers improves.
D)   The plant's reliance on photosynthesis reduces.E) A) and B)
F) A) and C)

Question 13

Match each term with the most appropriate macromolecule being studied or manipulate
-microarray

Accepted Answer

A)  DNA
B)  mRNA/cDNA
C)  protein
D)   protein.E) B) and C)
F) A) and D)

Question 14

Match each term with the most appropriate macromolecule being studied or manipulate
-proteomics

Accepted Answer

A)  DNA
B)  mRNA/cDNA
C)  protein
D)   DNA.E) A) and D)
F) A) and B)

Question 15

Where is cloned DNA in bacteria usually found?

Accepted Answer

A)   on a virus
B)   on the flagellum
C)   on a plasmid
D)   on the bacterial chromosomeE) B) and C)
F) A) and C)

Question 16

Which of the following is needed in order to amplify the DNA for gene X and NOT for gene Y?

Accepted Answer

A)   a heat-stable DNA polymerase
B)   restriction endonucleases that cut gene X and not gene Y
C)   plasmid vectors that have multiple antibiotic resistance genes
D)   some prior knowledge of the sequence of gene X such that appropriate primers can be designedE) A) and D)
F) B) and C)

Question 17

What is the main difference between germ-line gene therapy and somatic gene therapy?

Accepted Answer

A)   Only germ-line gene therapy will have results limited to the current generation.
B)   Only somatic gene therapy can result in the modified genes being passed to the next generation.
C)   Only germ-line gene therapy results in altered gametes.
D)   Only the germ-line cells are altered in somatic gene therapy.E) None of the above
F) A) and B)

Question 18

Genetically modified crops have been banned in Canada.

Accepted Answer

A) True 
 B)False

Question 19

How many sources of DNA sequences are commonly used in biotechnology?

Accepted Answer

A)   1
B)   2
C)   3
D)   4E) All of the above
F) A) and C)

Question 20

Which of the following is like a set of clones that collectively contains a copy of every DNA sequence in a genome?

Accepted Answer

A)   restriction enzyme library
B)   cloning vector
C)   genomic DNA library
D)   cDNA libraryE) None of the above
F) C) and D)

Exam 14: Dna Technologies

Which of the following best describes the purpose of creating transgenic mice?

Correct AnswerverifiedShow Answer

Which of the following describes biotechnology?

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Suppose that you have cloned a genomic library and know that the bacteria have foreign DNA fragments inserted into the cloning vector. Also suppose that you need to identify the specific bacteria that have the gene coding for protein X. How can these bacteria be located?

Correct AnswerverifiedShow Answer

Match each term with the most appropriate macromolecule being studied or manipulate -proteomics

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Which of the following is needed in order to amplify the DNA for gene X and NOT for gene Y?

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Match each term with the most appropriate macromolecule being studied or manipulate -microarray

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Figure 14-1 -According to Figure 14-1, which of the following is the difference between the original plasmid and the recombinant plasmid?

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Suppose that you generate cDNA libraries for the same cell line under different conditions. Also suppose that when you compare the libraries, you find that some cDNAs in one library are missing from the other library. What is the best explanation for this result?

Correct AnswerverifiedShow Answer

Genetically modified crops have been banned in Canada.

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How many sources of DNA sequences are commonly used in biotechnology?

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Match each term with its definition. -systems biology

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What is the main difference between germ-line gene therapy and somatic gene therapy?

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How are the sticky ends obtained?

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Match each term with the most appropriate macromolecule being studied or manipulate -cloning

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Which of the following is like a set of clones that collectively contains a copy of every DNA sequence in a genome?

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Why is Southern blotting used?

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Match each term with its definition. -transgenic

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What is a benefit of introducing new genes into a plant?

Correct AnswerverifiedShow Answer

Match the names of the processes to the descriptions. A process may be used once, more than once, or not at all. -This technique is used to amplify specific sequences of DNA.

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Where is cloned DNA in bacteria usually found?

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