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Exam 2: Tools of the Laboratory: Methods for the Culturing and Microscopic Analysis of Microorganisms

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Question 31

Multiple Choice

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Which of the following is not an inoculating tool?

A) Petri dish
B) Loop
C) Needle
D) Pipette
E) Swab

F) All of the above
G) A) and E)

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Question 32

Multiple Choice

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A nutrient medium that contains at least one ingredient that is NOT chemically definable would be termed ________.

A) complex
B) reducing
C) enriched
D) synthetic
E) minimal

F) A) and E)
G) A) and D)

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Question 33

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Which of the following characteristics refers to the microscope's ability to show two separate entities as separate and distinct?

The procedures for culturing a microorganism require the use of a microscope.

The all-purpose media Tryptic Soy Agar (TSA) contains the following four ingredients; pancreatic digest of casein,digest of soybean meal,NaCl and agar.  TSA is best described as ________.

Basic dyes are ________.

The bending of light rays as they pass from one medium to another is called refraction.

The patient is diagnosed with a bacterial infection after gram-positive cocci are detected in the fluid sample.The Gram stain involves ________.

Which type of microscope is the most widely used and shows cells against a bright background?

Agar is a complex polysaccharide that comes from a(n) ________.

Fixed smears of specimens are required in order to perform the Gram stain and endospore stain on the specimens.

A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium.Following incubation,only the E.coli grows in the culture.What is the most likely explanation?

A selective medium contains one or more substances that inhibit growth of certain microbes in order to facilitate the growth of other microbes.

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.All the cells appear blue under the oil lens.This is an example of ________.

Agar is an important component of media because ________.

Which of the following magnifies the specimen to produce its real image?

A 65-year-old homeless male presents to an urgent care clinic with a deep laceration on his left arm. He states that he cut his arm on an old piece of scrap metal two days prior. His wound is red, tender, hot to the touch, and has yellow drainage. The RN collects a sample of the drainage and sends it to the laboratory for microbial analysis, per provider orders. -Cultures and specimens pose a potential health hazard and require proper handling and disposal via specific medical waste policies.Some facilities are regulated to maintain living catalogs of specimens that may be subcultured into a fresh medium for research and educational purposes.Such collections are referred to as ________.

The ________ of the microscope holds and allows selection of the objective lenses.

The type of microscope in which you would see brightly illuminated specimens against a black background is ________.

Bacterial cultures are easily identified from their microscopic appearance.