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Which of the following represents the true significance of the finding that the cyanelles of P. chromatophora stem from a different type of cyanobacterium than gave rise to chloroplasts?


A) This finding indicates that there is a second evolutionary lineage of photosynthetic eukaryotes.
B) This finding represents the first time that primary endosymbiosis has been directly observed.
C) This finding is the strongest evidence yet for the theory of endosymbiosis.
D) This finding is an example of the phenomenon known as "serial endosymbiosis."
E) This finding is the first evidence that eukaryotic cells do not necessarily digest prokaryotic cells that manage to gain access to their cytoplasm.

F) C) and D)
G) A) and D)

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The evolution of multicellularity in animals has primarily occurred by the


A) remodeling of old genes.
B) deletion of many old genes.
C) production of many new genes.
D) deletion of many old chromosomes.
E) fusion of genes from many ancestral organisms.

F) B) and D)
G) All of the above

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The next few questions refer to the following description. Healthy individuals of Paramecium bursaria contain photosynthetic algal endosymbionts of the genus Chlorella. When within their hosts, the algae are referred to as zoochlorellae. In aquaria with light coming from only one side, P. bursaria gathers at the well-lit side, whereas other species of Paramecium gather at the opposite side. The zoochlorellae provide their hosts with glucose and oxygen, and P. bursaria provides its zoochlorellae with protection and motility. P. bursaria can lose its zoochlorellae in two ways: (1) if kept in darkness, the algae will die; and (2) if prey items (mostly bacteria) are absent from its habitat, P. bursaria will digest its zoochlorellae. -Which term most accurately describes the nutritional mode of healthy P. bursaria?


A) photoautotroph
B) photoheterotroph
C) chemoheterotroph
D) chemoautotroph
E) mixotroph

F) C) and D)
G) A) and B)

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The closest living relative of P. chromatophora is the heterotroph P. ovalis. P. ovalis uses threadlike pseudopods to capture its prey, which it digests internally. Which of the following, if observed, would be the best reason for relabeling P. chromatophora as a mixotroph?


A) a threadlike pseudopod
B) a pigmented central vacuole, surrounded by a tonoplast
C) a vacuole with food inside
D) a secretory vesicle
E) a contractile vacuole

F) None of the above
G) A) and B)

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Judging from Table 25.1 and given that water's density and, consequently, its buoyancy decrease at warmer temperatures, in which environment should diatoms (and other suspended particles) sink most slowly?


A) cold fresh water
B) warm fresh water
C) cold seawater
D) warm seawater
E) warm brackish water

F) C) and D)
G) B) and E)

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During passage through the large intestine, a trophozoite will often secrete a case around itself, forming a cyst. Cysts contain four haploid nuclei. When cysts "hatch" within a new host, two trophozoites are released. Thus, which of the following must happen within the cyst, prior to hatching? 1. meiosis 2) nuclear division 3) S phase 4) binary fission


A) 1 only
B) 1 and 2
C) 2 and 3
D) 2 and 4
E) 2, 3, and 4

F) A) and B)
G) A) and C)

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You are given five test tubes, each containing an unknown protist, and your task is to read the following description and match these five protists to the correct test tube. In test tube 1, you observe an organism feeding. Your sketch of the organism looks very similar to Figure 25.1. When light, especially red and blue light, is shone on the tubes, oxygen bubbles accumulate on the inside of test tubes 2 and 3. Chemical analysis of test tube 3 indicates the presence of substantial amounts of silica. Chemical analysis of test tube 2 indicates the presence of a chemical that is toxic to fish and humans. Microscopic analysis of organisms in test tubes 2, 4, and 5 reveals the presence of permanent, membrane-bounded sacs just under the plasma membrane. Microscopic analysis of organisms in test tube 4 reveals the presence of an apicoplast in each. Microscopic analysis of the contents in test tube 5 reveals the presence of one large nucleus and several small nuclei in each organism. You are given five test tubes, each containing an unknown protist, and your task is to read the following description and match these five protists to the correct test tube. In test tube 1, you observe an organism feeding. Your sketch of the organism looks very similar to Figure 25.1. When light, especially red and blue light, is shone on the tubes, oxygen bubbles accumulate on the inside of test tubes 2 and 3. Chemical analysis of test tube 3 indicates the presence of substantial amounts of silica. Chemical analysis of test tube 2 indicates the presence of a chemical that is toxic to fish and humans. Microscopic analysis of organisms in test tubes 2, 4, and 5 reveals the presence of permanent, membrane-bounded sacs just under the plasma membrane. Microscopic analysis of organisms in test tube 4 reveals the presence of an apicoplast in each. Microscopic analysis of the contents in test tube 5 reveals the presence of one large nucleus and several small nuclei in each organism.   Figure 25.1 The next few questions refer to the following description and Figure 25.1. -Test tube 2 contains A)  Paramecium. B)  Navicula (diatom) . C)  Pfiesteria (dinoflagellate) . D)  Entamoeba. E)  Plasmodium. Figure 25.1 The next few questions refer to the following description and Figure 25.1. -Test tube 2 contains


A) Paramecium.
B) Navicula (diatom) .
C) Pfiesteria (dinoflagellate) .
D) Entamoeba.
E) Plasmodium.

F) A) and B)
G) A) and C)

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The chloroplasts of all of the following are thought to be derived from ancestral red algae, except those of


A) golden algae.
B) diatoms.
C) dinoflagellates.
D) green algae.
E) brown algae.

F) A) and B)
G) A) and C)

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Unlike most excavates, Giardia trophozoites have no oral groove and are unable to form food vacuoles. Thus, we should expect its nutrition (mostly glucose) to come from


A) its mitosomes.
B) endosymbiotic cyanobacteria.
C) the ventral disk by which it adheres to the intestinal lining.
D) osmosis involving aquaporins.
E) plasma membrane proteins that are transporters or pumps.

F) B) and E)
G) C) and D)

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Which two genera have members that can evade the human immune system by frequently changing their surface proteins? 1. Plasmodium 2) Trichomonas 3) Paramecium 4) Trypanosoma 5) Entamoeba


A) 1 and 2
B) 1 and 4
C) 2 and 3
D) 2 and 4
E) 4 and 5

F) B) and E)
G) A) and C)

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Based on the phylogenetic tree in the figure given below, which of the following statements is correct? Based on the phylogenetic tree in the figure given below, which of the following statements is correct?   A)  The most recent common ancestor of Excavata is older than that of the SAR clade. B)  The most recent common ancestor of the SAR clade is older than that of Unikonta. C)  The most recent common ancestor of red algae and land plants is older than that of nucleariids and fungi. D)  The most basal (first to diverge)  eukaryotic supergroup cannot be determined. E)  Excavata is the most basal eukaryotic supergroup.


A) The most recent common ancestor of Excavata is older than that of the SAR clade.
B) The most recent common ancestor of the SAR clade is older than that of Unikonta.
C) The most recent common ancestor of red algae and land plants is older than that of nucleariids and fungi.
D) The most basal (first to diverge) eukaryotic supergroup cannot be determined.
E) Excavata is the most basal eukaryotic supergroup.

F) A) and E)
G) A) and C)

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Paulinella chromatophora is one of the few cercozoans that is autotrophic, carrying out aerobic photosynthesis with its two elongated "cyanelles." The cyanelles are contained within vesicles of the host cell, and each is derived from a cyanobacterium, though not the same type of cyanobacterium that gave rise to the chloroplasts of algae and plants. -What must occur for asexual reproduction to be successful in P. chromatophora? 1. mitosis 2) S phase 3) meiosis 4) equal distribution of cyanelles during cytokinesis


A) 1 only
B) 1 and 2
C) 1, 2, and 3
D) 1, 2, and 4
E) 2, 3, and 4

F) A) and B)
G) B) and D)

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The next few questions refer to the following description. Healthy individuals of Paramecium bursaria contain photosynthetic algal endosymbionts of the genus Chlorella. When within their hosts, the algae are referred to as zoochlorellae. In aquaria with light coming from only one side, P. bursaria gathers at the well-lit side, whereas other species of Paramecium gather at the opposite side. The zoochlorellae provide their hosts with glucose and oxygen, and P. bursaria provides its zoochlorellae with protection and motility. P. bursaria can lose its zoochlorellae in two ways: (1) if kept in darkness, the algae will die; and (2) if prey items (mostly bacteria) are absent from its habitat, P. bursaria will digest its zoochlorellae. -Which term best describes the symbiotic relationship of well-fed P. bursaria to their zoochlorellae?


A) mutualistic
B) commensal
C) parasitic
D) predatory
E) pathogenic

F) A) and C)
G) A) and D)

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The last common ancestor of fungi and animals was most likely a


A) colonial photosynthetic eukaryote.
B) multicellular photosynthetic eukaryote.
C) colonial photosynthetic prokaryote.
D) multicellular photosynthetic prokaryote.
E) single-celled eukaryote.

F) A) and E)
G) B) and E)

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The cysts of Giardia are most analogous to the


A) mitochondria of ancestral diplomonads.
B) nuclei of archaeans.
C) endospores of bacteria.
D) capsids of viruses.

E) A) and C)
F) None of the above

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The next few questions refer to the following description. Healthy individuals of Paramecium bursaria contain photosynthetic algal endosymbionts of the genus Chlorella. When within their hosts, the algae are referred to as zoochlorellae. In aquaria with light coming from only one side, P. bursaria gathers at the well-lit side, whereas other species of Paramecium gather at the opposite side. The zoochlorellae provide their hosts with glucose and oxygen, and P. bursaria provides its zoochlorellae with protection and motility. P. bursaria can lose its zoochlorellae in two ways: (1) if kept in darkness, the algae will die; and (2) if prey items (mostly bacteria) are absent from its habitat, P. bursaria will digest its zoochlorellae. -The motility that permits P. bursaria to move toward a light source is provided by


A) pseudopods.
B) a single flagellum composed of the protein flagellin.
C) a single flagellum featuring the 9 + 2 pattern.
D) many cilia.
E) contractile vacuoles.

F) B) and C)
G) B) and E)

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Reinforced, threadlike pseudopods that can perform phagocytosis are generally characteristic of which group(s) ?


A) radiolarians and forams
B) gymnamoebas
C) entamoebas
D) amoeboid stage of cellular slime molds
E) oomycetes

F) A) and E)
G) B) and E)

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Which of the following statements about dinoflagellates is true?


A) They possess two flagella.
B) All known varieties are autotrophic.
C) Their walls are usually composed of silica plates.
D) Many types lack mitochondria.
E) Their dead cells accumulate on the seafloor, and are mined to serve as a filtering material.

F) A) and B)
G) B) and E)

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The closest living relative of P. chromatophora is the heterotroph P. ovalis. What type of evidence permits biologists to make this claim about relatedness?


A) morphological
B) ecological
C) biochemical
D) genetic
E) fossil

F) A) and C)
G) A) and D)

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If true, which of the following would be most important in determining whether P. chromatophora's cyanelle is still an endosymbiont, or is an organelle, as the term cyanelle implies?


A) if P. chromatophora is less fit without its cyanelle than with it
B) if the cyanelle is less fit without the host cercozoan than with it
C) if there is ongoing metabolic cooperation between the cyanelle and the host cercozoan
D) if the magnesium-containing porphyrin ring in the cyanelle's chlorophyll molecules is built by the cyanelle, whereas the organic portion of the chlorophyll molecules is built by the host cercozoan
E) if there has been movement of genes from the cyanelle genome to the nuclear genome, such that these genes are no longer present in the cyanelle genome

F) A) and E)
G) A) and B)

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