Question 1

If the following DNA strand were used as a template, what would the sequence of an RNA be? 5' GTACCGTC 3'

Accepted Answer

A)  5'GUACCGUC 3'
B)  5'GACGGTAC 3'
C)  5' CAUGGCAG 3'
D)  5' GACGGUAC 3'
E)  5' GUCGGUAC 3'F) B) and E)
G) C) and D)

Question 2

Which of the following is NOT necessary for RNA polymerase to recognize the promoter of a bacterial gene?

Accepted Answer

A)  Sigma factor
B)  Origin of replication
C)  -10 consensus sequence
D)  -35 consensus sequenceE) B) and D)
F) B) and C) B

Question 3

What would you add to an in vitro transcription system that contains an E.coli gene in order to get transcription that begins from the normal transcription start site?

Accepted Answer

(1)RNA polymerase (2)Sigma factor (3)Ribonucleoside triphosphates with A, C, G, and U bases (rNTPs)

Question 4

Which of the following statements about bacterial mRNA transcript is TRUE?

Accepted Answer

A)  Unlike eukaryotes, bacterial mRNA transcripts do not typically contain untranslated regions.
B)  The Shine-Dalgarno box associates with an RNA component in the ribosomes.
C)  Transcription and translation take place sequentially in bacterial cells.
D)  Most bacterial genes contain a large number of introns and small number of exons.
E)  The 5' end and 3' end of mRNA transcripts are modified in bacteria.F) B) and E)
G) A) and C)

Question 5

Which process is illustrated in the diagram below?

Accepted Answer

A)  Transcription
B)  Translation
C)  RNA processing
D)  Replication
E)  Nucleosome assemblyF) B) and D)
G) C) and D)

Question 6

What are the three different RNA polymerases found in all eukaryotes, and what types of genes do they transcribe?

Accepted Answer

(1)RNA polymerase I transcribes large rRNA. (2)RNA polymerase II transcribes pre-mRNA, some snRNA, snoRNAs, some miRNAs. (3)RNA polymerase III transcribes tRNA, small rRNA, some snRNAs, and some miRNAs.

Question 7

Which of the following statements is TRUE regarding transcription in most organisms?

Accepted Answer

A)  All genes are transcribed from the same strand of DNA.
B)  Both DNA strands are used to transcribe a single gene.
C)  Different genes may be transcribed from different strands of DNA.
D)  The DNA template strand is used to encode double-stranded RNA.
E)  The DNA nontemplate strand is used to encode single-stranded RNA.F) B) and E)
G) A) and B)

Question 8

Anticodons are found on _____ molecules.

Accepted Answer

A)  mRNA
B)  tRNA
C)  rRNA
D)  snRNA
E)  miRNAF) A) and B)
G) A) and C)

Question 9

Which of the following is a sequence of DNA where transcription is initiated?

Accepted Answer

A)  Hairpin loop
B)  Terminator
C)  Initiator
D)  Sigma factor
E)  PromoterF) B) and E)
G) None of the above

Question 10

Whereas the nucleotide strand used for transcription is termed the _____, the nontranscribed strand is called the _____.

Accepted Answer

A)  promoter; terminator
B)  terminator; promoter
C)  transcription apparatus; TATA box
D)  template strand; nontemplate strand
E)  nontemplate strand; template strandF) B) and E)
G) B) and D)

Question 11

Where are promoters usually located?

Accepted Answer

A)  Upstream of the start site
B)  Downstream of the start site
C)  Near nucleotide +25
D)  Near the hairpin loop
E)  Downstream of the terminatorF) B) and D)
G) A) and B)

Question 12

Which of the following small RNA types is unique to prokaryotes?

Accepted Answer

A)  siRNA
B)  crRNA
C)  miRNA
D)  piRNA
E)  lncRNAF) C) and D)
G) D) and E)

Question 13

The 5' cap on an mRNA is important for all the processes listed below EXCEPT for the _____ of an mRNA molecule.

Accepted Answer

A)  transcription
B)  intron removal
C)  stability
D)  initiation of translationE) A) and B)
F) None of the above

Question 14

Which mechanism allows for more than one polypeptide to be encoded by a single gene?

Accepted Answer

A)  Regulated transcription
B)  RNA interference
C)  Alternative RNA processing
D)  Self-splicing of introns
E)  RNA methylationF) A) and D)
G) B) and C)

Question 15

When does sigma factor normally dissociate from RNA polymerase?

Accepted Answer

A)  After transcription has terminated
B)  After the process of initiation
C)  After the addition of nucleosomes
D)  After the binding of rho
E)  Following the addition of nucleosomesF) A) and B)
G) None of the above

Question 16

Scientists once believed that each gene can encode a single polypeptide.We now know that _____ and _____ allow a single gene to encode more than one polypeptide.

Accepted Answer

A)  transcription; translation
B)  polyadenylation; RNA transport
C)  DNA methylation; chromatin condensation
D)  alternative processing; RNA editing
E)  gene silencing; RNA interferenceF) A) and B)
G) A) and C)

Question 17

Which of the following is NOT required for transcription?

Accepted Answer

A)  Ribonucleotides
B)  RNA primers
C)  DNA template
D)  RNA polymerase
E)  PromoterF) A) and D)
G) A) and C)

Question 18

What types of bonds are created between nucleotides during the process of transcription?

Accepted Answer

A)  Ionic
B)  Oxygen
C)  Phosphodiester
D)  Hydrogen
E)  Both phosphodiester and hydrogen are correct.F) A) and C)
G) B) and C)

Question 19

Which of the following RNA molecules are required for the process of translation?

Accepted Answer

A)  crRNA
B)  tRNA
C)  snRNA
D)  miRNA
E)  siRNAF) A) and B)
G) B) and C)

Question 20

In prokaryotic RNA polymerases, the holoenzyme consists of the core enzyme and the:

Accepted Answer

A)  rho factor.
B)  TFIID.
C)  TBP.
D)  omega subunit.
E)  sigma factor.F) A) and C)
G) B) and E)

Exam 10: From DNA to Proteins- Transcription and Rna Processing

If the following DNA strand were used as a template, what would the sequence of an RNA be? 5' GTACCGTC 3'

Correct AnswerverifiedShow Answer

Which of the following is NOT necessary for RNA polymerase to recognize the promoter of a bacterial gene?

Correct AnswerverifiedB

What would you add to an in vitro transcription system that contains an E.coli gene in order to get transcription that begins from the normal transcription start site?

Correct Answerverified(1)RNA polymerase (2)Sigma factor (3)Ribonucleoside triphosphates with A, C, G, and U bases (rNTPs)

Which of the following statements about bacterial mRNA transcript is TRUE?

Correct AnswerverifiedShow Answer

Which process is illustrated in the diagram below?

Correct AnswerverifiedShow Answer

What are the three different RNA polymerases found in all eukaryotes, and what types of genes do they transcribe?

Correct Answerverified(1)RNA polymerase I transcribes large rRNA. (2)RNA polymerase II transcribes pre-mRNA, some snRNA, snoRNAs, some miRNAs. (3)RNA polymerase III transcribes tRNA, small rRNA, some snRNAs, and some miRNAs.

Which of the following statements is TRUE regarding transcription in most organisms?

Correct AnswerverifiedShow Answer

Anticodons are found on _____ molecules.

Correct AnswerverifiedShow Answer

Which of the following is a sequence of DNA where transcription is initiated?

Correct AnswerverifiedShow Answer

Whereas the nucleotide strand used for transcription is termed the _____, the nontranscribed strand is called the _____.

Correct AnswerverifiedShow Answer

Where are promoters usually located?

Correct AnswerverifiedShow Answer

Which of the following small RNA types is unique to prokaryotes?

Correct AnswerverifiedShow Answer

The 5' cap on an mRNA is important for all the processes listed below EXCEPT for the _____ of an mRNA molecule.

Correct AnswerverifiedShow Answer

Which mechanism allows for more than one polypeptide to be encoded by a single gene?

Correct AnswerverifiedShow Answer

When does sigma factor normally dissociate from RNA polymerase?

Correct AnswerverifiedShow Answer

Scientists once believed that each gene can encode a single polypeptide.We now know that _____ and _____ allow a single gene to encode more than one polypeptide.

Correct AnswerverifiedShow Answer

Which of the following is NOT required for transcription?

Correct AnswerverifiedShow Answer

What types of bonds are created between nucleotides during the process of transcription?

Correct AnswerverifiedShow Answer

Which of the following RNA molecules are required for the process of translation?

Correct AnswerverifiedShow Answer

In prokaryotic RNA polymerases, the holoenzyme consists of the core enzyme and the:

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified
B

Correct Answer
verified
(1)RNA polymerase (2)Sigma factor (3)Ribonucleoside triphosphates with A, C, G, and U bases (rNTPs)

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
(1)RNA polymerase I transcribes large rRNA. (2)RNA polymerase II transcribes pre-mRNA, some snRNA, snoRNAs, some miRNAs. (3)RNA polymerase III transcribes tRNA, small rRNA, some snRNAs, and some miRNAs.

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Whereas the nucleotide strand used for transcription is termed the _, the nontranscribed strand is called the _.

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Scientists once believed that each gene can encode a single polypeptide.We now know that _ and _ allow a single gene to encode more than one polypeptide.

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified